Arthritis & Rheumatism, Volume 62,
November 2010 Abstract Supplement
Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Atlanta, Georgia November 6-11, 2010.
Decreased TH17 Cells in the Peripheral Blood of Patients with Early Spondyloarthritis (ESpA).
Bautista-Caro2, María Belén, Arroyo-Villa2, Irene, Castillo-Gallego2, Concepcion, de Miguel-Mendieta2, Eugenio, Martin-Mola2, Emilio, Miranda-Carus1, María Eugenia
Th17 cells may be implicated in the pathogenesis of Spondyloarthritides (SpA). Previous data regarding the percentage of Th17 cells in the peripheral blood of Early SpA (ESpA) patients are limited and contradictory.
To determine the frequency and phenotype of Th17 cells in the peripheral blood of patients with ESpA.
We studied 22 healthy controls and 22 patients with ESpA (who met ESSG or other accepted SpA Classification Criteria) from the "Esperanza" programme of the Spanish Rheumatology Foundation. The inclusion criteria were inflammatory back pain or asymmetric peripheral arthritis, duration of disease of three to 24 months and age of 18 to 45 years. At baseline visit, medical history, physical examination, ESR, CRP, HLAB27 and pelvis conventional x-Ray were recorded.
CD4 T cells were magnetically sorted from freshly isolated peripheral blood and cultured overnight in the presence of PMA and ionomycin. IL-17 was determined by ELISA of culture supernatants and by flow cytometry of monensin-treated cells. In addition the expression of CCR6, IL-23R and RORgT were examined by flow cytometry.
ESpA patients were 7 females, 15 males. Age was (mean ± SD) 36 ± 7 years, duration of disease 17 ± 10 months. BASDAI index was 3.7 ± 2.2 (median 3.55, range 1.28.3) and BASFI was 1.82 ± 1.69 (median 1.65, range 0.36.1). CD4 T cells of ESpA patients secreted signficantly lower amounts of IL-17 into the culture medium (1134.9 ± 488.6, median 687.97, range 99.023156.33) when compared with controls (2059.3 ± 664.6 pg/ml, median 1719.67, range 448.116243.9), p<0.05. Likewise, the percentage of IL-17 producing CD4 T cells (Th17 cells) was significantly lower in ESpA patients: 0.93 ± 0.26, median 0.84, range 0.222.15 in patients vs 1.50 ± 0.56,median1.13, range 0.663.92 in controls, p< 0.05. Th17 cells expressed CCR6, RORgT and IL-23R. Disease activity as assesed by BASDAI index was negatively correlated with IL-17 concentration and with the percentage of Th17 cells. Likewise, BASFI function index showed a negative correlation with soluble IL-17 and with the percentage of Th17 cells.
Peripheral blood CD4 T cells from ESpA patients demonstrate a decreased secretion capacity of IL-17 together with decreased percentage of Th17 cells, that are related with disease activity as assessed by BASDAI index and with the functional index BASFI.
To cite this abstract, please use the following information:
Bautista-Caro, María Belén, Arroyo-Villa, Irene, Castillo-Gallego, Concepcion, de Miguel-Mendieta, Eugenio, Martin-Mola, Emilio, Miranda-Carus, María Eugenia; Decreased TH17 Cells in the Peripheral Blood of Patients with Early Spondyloarthritis (ESpA). [abstract]. Arthritis Rheum 2010;62 Suppl 10 :1967