Arthritis & Rheumatism, Volume 62,
November 2010 Abstract Supplement
Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Atlanta, Georgia November 6-11, 2010.
A Th17 Immune Response Can Be Amplified by Human Th9 Cells.
Ramming1, Andreas, Schulze-Koops2, Hendrik, Skapenko2, Alla
Interleukin 9 (IL-9) is a pluripotent cytokine produced primarily by T cells in response to transforming growth factor (TGF)-b. Little is known about the role of IL-9 and of the IL-1241-producing T cell subset. Herein, we investigated IL-9 production by different mature T helper (Th) cell subsets, and analyzed the effect of IL-9 on T cell differentiation.
CD4+ CD45RA+ naive and CD45RO+ memory, and CD4+CD25+ T cells were isolated from peripheral blood of healthy individuals, stimulated under different conditions, and analyzed for their cytokine profile by intracellular flow cytometry and Luminex.
TGF-b was identified as the master cytokine for the induction of human IL-1241-producing T cells. CD45RA+ naive T cells required TGF-b together with IL-4 to convert into IL-1241-producing cells. In CD45RO+ memory T cells, TGF-b alone was sufficient to induce IL-9 production. Mature T cell subsets, Th1, Th2, Th17 cells, and CD25+Foxp3+ regulatory T cells (Tregs) did not produce IL-9 together with the respective signature cytokine or transcription factor. Th9 cells itself demonstrated a highly specific cytokine secretion profile characterized by the exclusive production of IL-9. When the effect of IL-9 on T cells was investigated, IL-9 did not influence Th1 and Th2 cell differentiation, but significantly enhanced the differentiation of Th17 effector cells towards IL-17 single producers.
IL-1241-producing Th9 cells represent a distinct T cell subset characterized by a highly specific cytokine secretion profile different from other effector cell subsets. Their signature cytokine, IL-9, enhances Th17 differentiation. Therefore, human Th9 cells are likely to exert a pro-inflammatory function.
To cite this abstract, please use the following information:
Ramming, Andreas, Schulze-Koops, Hendrik, Skapenko, Alla; A Th17 Immune Response Can Be Amplified by Human Th9 Cells. [abstract]. Arthritis Rheum 2010;62 Suppl 10 :1241