Arthritis & Rheumatism, Volume 62,
November 2010 Abstract Supplement

Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Atlanta, Georgia November 6-11, 2010.

Synovial Extracellular Matrix Remodeling in Experimental Model of Diabetes.

Atayde1,  Sandra Aparecida, Andrade2,  Priscila Cristina, Catanozi2,  Sérgio, Nakandakare2,  Edna Regina, Passarelli2,  Marisa, Velosa2,  Ana Paula Pereira, Capelozzi2,  Vera Luiza

University of São Paulo, São Paulo, Brazil
University of São Paulo


Diabetes mellitus has been linked to disorders of bones and joints whose etiologic mechanisms could be linked to micro vascular disease. The synovia is the most vascularized tissue in the joint, therefore, can be made more resilient to endothelial injury in diabetic process. Our purpose was to evaluate the extracellular matrix components and specially the vascular lesion in this tissue after diabetes induction in rats.


Wistar young rats, were divided into diabetic group (DG; n=15) and control group (CG; n=15). Diabetes was induced by tail injection of streptozotocin (35mg/kg), while control group received saline solution. Weight, blood glucose and plasmatic anti-carboxymethyl lysine analysis had been done after 70 days. After euthanasia, knee joints were isolated, and included in paraffin. Histological sections stained with Hematoxilin-Eosin and Picrosirius were obtained for histomorphometric analysis. Total collagen quantification of this tissue was done in Picrosirius under polarized light. The distribution of collagens I, III and V and also carboxymethyl lysine receptor were assessed by immunofluorescence and immunochemistry was used for endothelin -1 detection in synovial tissue.


Higher levels of blood glucose and plasmatic anti-carboxymethyl lysine was viewed in DG when compared to CG (p<0,05). Moreover the final weight was lower in DG than CG(p<0,05). Histomorphometric analysis showed increased of thick collagen content and decrease of thin collagen fibers in synovial tissue. Immunofluorescence test demonstrated increase of collagen I expressions in synovial tissue of DG. The endothelin -1 and carboxymethyl lysine receptor were highly expressed in DG synovial tissue.


There were intense remodeling and collagen deposition in the synovia of animals with diabetes induced by streptozotocin, which may explain the pathophysiology of musculoskeletal dysfunctions observed in diabetic patients.

To cite this abstract, please use the following information:
Atayde, Sandra Aparecida, Andrade, Priscila Cristina, Catanozi, Sérgio, Nakandakare, Edna Regina, Passarelli, Marisa, Velosa, Ana Paula Pereira, et al; Synovial Extracellular Matrix Remodeling in Experimental Model of Diabetes. [abstract]. Arthritis Rheum 2010;62 Suppl 10 :887
DOI: 10.1002/art.28655

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