Arthritis & Rheumatism, Volume 62,
November 2010 Abstract Supplement
Abstracts of the American College of
Rheumatology/Association of Rheumatology Health Professionals
Annual Scientific Meeting
Atlanta, Georgia November 6-11, 2010.
Lack of Detection of Xenotropic Murine Leukemia Virus-Related Virus (XMRV) in Patients with Fibromyalgia.
Martin1, Jorge J. Gonzalez, Herrero-Beaumont2, Gabriel, Delgado3, Rafael
Xenotropic murine leukemia virus-related virus (XMRV) is a recently described human retrovirus that has been associated with prostate cancer. Recently, XMRV sequences and specific immune responses have been detected in 67% of patients with the diagnosis of Chronic Fatigue Syndrome (CFS) in North America. Based in the common features between CFS and Fibromyalgia (FM), we decide to study the presence of XMRV in a group of 15 patients previously diagnosed with FM. DNA was extracted from whole blood and analyzed by nested PCR using 3 sets of primers corresponding to the gag and env regions of XMRV. Primers for human beta-globin (hBG) were used as positive control of human DNA amplification. The sensitivity of the nested PCR procedure, estimated by spiking the XMRV molecular clone VP62 into negative samples, was 110 copies per sample. We did not found any evidence of this new agent in any patient with FM studied and XMRV does not appear to be related with FM. With this relative small population we cannot absolutely exclude an association of XMRV with FM, however it would be highly unlikely a proportion of FM cases with XMRV greater than 22% (3 of 15 cases, 95% confidence interval), which is clearly insufficient to support a significant association between XMRV and FM.
To cite this abstract, please use the following information:
Martin, Jorge J. Gonzalez, Herrero-Beaumont, Gabriel, Delgado, Rafael; Lack of Detection of Xenotropic Murine Leukemia Virus-Related Virus (XMRV) in Patients with Fibromyalgia. [abstract]. Arthritis Rheum 2010;62 Suppl 10 :804