Arthritis & Rheumatism, Volume 60,
October 2009 Abstract Supplement

The 2009 ACR/ARHP Annual Scientific Meeting
Philadelphia October 16-21, 2009.


Altered Expression of MiR-155 and MiR-181a in Periphery Blood Mononuclear Cells of Rheumatoid Arthritis

Long1,  Li, Shi1,  Jinxia, Li1,  Ru, Li2,  Zhanguo

Bejing Univ People's Hosp, Beijing, China
Peking University People's Hospital, Beijing, China

Purpose:

1. To Screen for the miRNAs differently expressed in Periphery Blood Mononuclear Cells (PBMCs) of RA by microarray experiments. 2. To further evaluate the expression of miR-155 and miR-181a in PBMCs of RA. 3. To determine the relevance between the expression of miR-155/miR181a and clinical as well as laboratory features. 4. To test whether inflammatory mediators can induce miR-155 and miR-181a in PBMCs of RA.

Method:

1. Total RNA was isolated from peripheral blood mononuclear cells obtained from 5 patients of RA and 5 normal controls. Expression profiling of miRNAs was performed in a microarray analysis. 2. MiR-155 and miR-181a were identified for further study by stem-loop real-time RT-PCR based on SYBR-Green. PBMC from 26 patients of RA and 23 normal controls were collected. 3. Associations between miR-155/miR-181a and the clinical and laboratory features of RA were evaluated. 4. Induction of miR-155 following stimulation with TNF-a, IFN-gand LPS of cultures of RA PBMCs was examined by real-time RT-PCR.

Results:

1. Expression profiling of miRNAs revealed significant differential expression of 46 miRNAs. MiR-155 was up-regulated in PBMC of RA than in normal controls, and miR-181a was down-regulated in RA group. 2. The expression level of miR-155 had a positive correlation with serum CRP level. 3. Expression of miR-155 was markedly up-regulated in PBMCs of RA after stimulation with TNF-a, IFN-g and LPS, especially with TNF-a. 4. Similar up-regulated expression of miR-155 upon stimuli was observed in healthy controls, however, RA PBMCs exhibit much higher expression of miR-155 in response to stimuli.

Conclusion:

MiRNA-155 may be a positive regulator in RA pathogenesis, while miRNA-181a may be a negative one. The expression of miR-155 is induced by stimulation with TNF-a, IFN-g and LPS. Further studies are required to elucidate the function of miR-155 and miR-181a.

To cite this abstract, please use the following information:
Long, Li, Shi, Jinxia, Li, Ru, Li, Zhanguo; Altered Expression of MiR-155 and MiR-181a in Periphery Blood Mononuclear Cells of Rheumatoid Arthritis [abstract]. Arthritis Rheum 2009;60 Suppl 10 :1647
DOI: 10.1002/art.26721

Abstract Supplement

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