Arthritis & Rheumatism, Volume 60,
October 2009 Abstract Supplement
The 2009 ACR/ARHP Annual Scientific Meeting
Philadelphia October 16-21, 2009.
Altered Expression of MiR-155 and MiR-181a in Periphery Blood Mononuclear Cells of Rheumatoid Arthritis
Long1, Li, Shi1, Jinxia, Li1, Ru, Li2, Zhanguo
1. To Screen for the miRNAs differently expressed in Periphery Blood Mononuclear Cells (PBMCs) of RA by microarray experiments. 2. To further evaluate the expression of miR-155 and miR-181a in PBMCs of RA. 3. To determine the relevance between the expression of miR-155/miR181a and clinical as well as laboratory features. 4. To test whether inflammatory mediators can induce miR-155 and miR-181a in PBMCs of RA.
1. Total RNA was isolated from peripheral blood mononuclear cells obtained from 5 patients of RA and 5 normal controls. Expression profiling of miRNAs was performed in a microarray analysis. 2. MiR-155 and miR-181a were identified for further study by stem-loop real-time RT-PCR based on SYBR-Green. PBMC from 26 patients of RA and 23 normal controls were collected. 3. Associations between miR-155/miR-181a and the clinical and laboratory features of RA were evaluated. 4. Induction of miR-155 following stimulation with TNF-a, IFN-gand LPS of cultures of RA PBMCs was examined by real-time RT-PCR.
1. Expression profiling of miRNAs revealed significant differential expression of 46 miRNAs. MiR-155 was up-regulated in PBMC of RA than in normal controls, and miR-181a was down-regulated in RA group. 2. The expression level of miR-155 had a positive correlation with serum CRP level. 3. Expression of miR-155 was markedly up-regulated in PBMCs of RA after stimulation with TNF-a, IFN-g and LPS, especially with TNF-a. 4. Similar up-regulated expression of miR-155 upon stimuli was observed in healthy controls, however, RA PBMCs exhibit much higher expression of miR-155 in response to stimuli.
MiRNA-155 may be a positive regulator in RA pathogenesis, while miRNA-181a may be a negative one. The expression of miR-155 is induced by stimulation with TNF-a, IFN-g and LPS. Further studies are required to elucidate the function of miR-155 and miR-181a.
To cite this abstract, please use the following information:
Long, Li, Shi, Jinxia, Li, Ru, Li, Zhanguo; Altered Expression of MiR-155 and MiR-181a in Periphery Blood Mononuclear Cells of Rheumatoid Arthritis [abstract]. Arthritis Rheum 2009;60 Suppl 10 :1647