Arthritis & Rheumatism, Volume 60,
October 2009 Abstract Supplement

The 2009 ACR/ARHP Annual Scientific Meeting
Philadelphia October 16-21, 2009.


Use of Laser Microdissection in the Analysis of Renal-Infiltrating T Cells in Murine Lupus

Wang1,  Yingge, Ito1,  Satoshi, Suziki1,  Mizuho, Sugihara1,  Makoto, Hayashi1,  Taichi, Goto1,  Daisuke, Matsumoto1,  Isao

University of Tsukuba, Tsukuba, Japan
Juntendo University School of Medicine, Tokyo, Japan

Purpose:

To clarify the role of T cells in the kidneys of murine lupus models, cytokine mRNA expression was analyzed, and tissue localization of T cells was examined by immunohistochemistry.

Method:

1) Cells infiltrating the glomeruli and perivascular areas in MRL/lpr (10 female), NZB/W F1 (B/WF1) (4 female), and BXSB (2 male) mice were captured by laser microdissection (LMD). 2) Nested reverse transcription polymerase chain reaction (RT-PCR) of samples was performed with primers specific for b-actin, T-cell receptor b chain (TCR-Cb), Thy-1, B220, CD4, CD8, interleukin (IL)-2, IL-4, IL-10, IL-13, IL-17, and interferon (IFN)-g. 3) Frozen sections of lesions were also stained immunohistochemically.

Results:

1) T cells infiltrating the glomeruli and perivascular areas produced IFN-g, IL-13, and IL-17 predominately. 2) IL-10 was detected only in the perivascular areas of MRL/lpr and B/W F1 mice but not in glomerulus. 3) Thy-1, CD4, CD8, B220, IFN-g, and IL-17 staining was observed in glomeruli and perivascular areas from MRL/lpr, B/W F1, and BXSB mice. However, IL-10 staining was observed only in perivascular areas from MRL/lpr and B/W F1 mice.

Conclusion:

Results of our study suggest that the cytokine balance is complex and not due simply to the Th1 and Th2 balance and IL-17 might play a critical role in murine lupus models. These findings also support the concept of different molecular mechanisms for glomerulonephritis and vasculitis in these mice.

To cite this abstract, please use the following information:
Wang, Yingge, Ito, Satoshi, Suziki, Mizuho, Sugihara, Makoto, Hayashi, Taichi, Goto, Daisuke, et al; Use of Laser Microdissection in the Analysis of Renal-Infiltrating T Cells in Murine Lupus [abstract]. Arthritis Rheum 2009;60 Suppl 10 :1036
DOI: 10.1002/art.26113

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