Arthritis & Rheumatism, Volume 60,
October 2009 Abstract Supplement
The 2009 ACR/ARHP Annual Scientific Meeting
Philadelphia October 16-21, 2009.
Aquaporin Expression in Interstitial Lung Diseases Related to Sjgren Syndrome
Navarro1, Carmen, Salgado2, Alfonso, Gaxiola3, Miguel, Mejia3, Mayra, Selman3, Moises
Aquaporins (AQP) are molecular water channels expressed in many epithelia and endothelia involved in fluid transport, such salival and lacrimal gland or lung. It has been shown down-regulation of AQP in salivary glands in patients with Sjögren's syndrome (SS) and altered expression on lacrimal gland in animal models of the disease suggesting AQP may play a crucial role in the pathology and clinical manifestations of the disease. To assess abnormalities in the expression of AQP in lung tissue of SS patients who develop interstitial lung disease (ILD).
We analyzed 7 lung biopsy obtained with diagnosis proposal from SS patients who develop ILD. Pattern of AQP 1, 4 and 5 mRNA expression in both, total lung tissue and epithelial cells obtained by laser captured microdissection, were examined by quantitative real-time PCR. We used RNA from normal lung commercial available (StratageneÒ, ClontechÒ, AmbionÒ) as control group.
AQP localization in lung tissue was evaluated by standard immunohistochemistry using antibodies commercial available (Santacruz Ò). Clinical data were collected from clinical charts.
The data we have obtained showed that 5 of the 7 SS-ILD patients had lower expression of AQP-1 than the average expression in the control group; 6 of the 7 SS-ILD patients had lower expression of AQP-4 than the control group; and all the patients had higher expression of AQP-5 than the control group. Similar results were found on the mRNA obtained from epithelial cells and total lung tissue. Localization of AQP-1 and AQP-4 were similar to normal tissue. However expression of AQP-5 was diminished compared with normal tissue.
Expression of AQP is modified in lung parenchyma of SS patients who develop interstitial lung diseases. Although the full implications of these findings remain to be determined, may be AQP could be involved in the lung damage of SS patients.
To cite this abstract, please use the following information:
Navarro, Carmen, Salgado, Alfonso, Gaxiola, Miguel, Mejia, Mayra, Selman, Moises; Aquaporin Expression in Interstitial Lung Diseases Related to Sjgren Syndrome [abstract]. Arthritis Rheum 2009;60 Suppl 10 :502