Arthritis & Rheumatism, Volume 60,
October 2009 Abstract Supplement
The 2009 ACR/ARHP Annual Scientific Meeting
Philadelphia October 16-21, 2009.
Relationship Between Foxp3 Tregs in Synovium of Rheumatoid Arthritis and Clinical Activity
Yan1, Zeng, Dong-hui1, Zheng, Lie1, Dai, Bai-yu1, Zhang, Ying-qian1, MO, Zhi-xun2, LI
CD4+CD25+Tregs play an important role to keep balance of immune system in patients with rheumatoid arthritis (RA). Foxp3 (forkhead box P3/winged-helix transcription factor), the prototypic transcription factor of Tregs, is not only specific but also critical to Tregs function. In this study, Foxp3 was used to identify Tregs and the relationship between synovial Foxp3+Tregs and clinical activity of RA was analyzed.
Synovium from active RA patients was taken by closed needle biopsy. Synovium from patients with OA and non-inflammatory orthopedic arthropathies was chosen as controls. Synovial serial sections were measured by HE staining and immunohistochemistry staining for Foxp3, CD3, CD20, CD34, CD38 and CD68. The cell count of the Foxp3+Tregs and the percentage of Foxp3+Tregs/CD3+Tcells were analyzed. The relationship between Foxp3+Tregs expression in RA synovium and the histological synovitis scores, clinical activity was analyzed. RA patients were divided into two groups according to the DAS28: intermediate active group (DAS28 3.2~5.1, n=12) and severe active group (DAS28 >5.1, n=13).
(1) Foxp3 was expressed in RA synovium, mainly in nuclei of CD3+T cells, and Foxp3 expression was negative in controls. (2)The cell count of Foxp3+Tregs and the percentage of Foxp3+Tregs/CD3+Tcells were less in severe RA group than those in the intermediate group(P=0.005, 0.001).(3)There was an inverse relationship between the cell count of Foxp3+Tregs and the synovitis scores, synovial hyperplasia, the percentage of CD68 positive cells in intimal lining layer (r=-0.622,-0.916,-0.935, P=0.001,0.000,0.000). (4)There was an inverse relationship between the cell count of Foxp3+Tregs in RA synovium and 28 tenderness joints' indexes or DAS28(r=-0.568,-0.656, P=0.003,0.000).
Foxp3+Tregs invasion in synovium may correlate with rheumatoid synovitis and clinical activity. Synovial Foxp3 expression may be a new diagnostic biomarker for clinical activity of RA patients.
To cite this abstract, please use the following information:
Yan, Zeng, Dong-hui, Zheng, Lie, Dai, Bai-yu, Zhang, Ying-qian, MO, Zhi-xun, LI; Relationship Between Foxp3 Tregs in Synovium of Rheumatoid Arthritis and Clinical Activity [abstract]. Arthritis Rheum 2009;60 Suppl 10 :391