Arthritis & Rheumatism, Volume 60,
October 2009 Abstract Supplement
The 2009 ACR/ARHP Annual Scientific Meeting
Philadelphia October 16-21, 2009.
ICAM-1 and VCAM-1 Concentrations in Synovial Fluid Correlate to the Degree of Synovitis Measured by MRI in Moderate to Severe Osteoarthritis of the Knee
Andersen1, Signe E.B., Jorgensen1, Annette, Ellingsen1, Torkell, Egund2, Niels, Stengaard-Pedersen1, Kristian
To quantify intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) in plasma and synovial fluid (SF) from patients with moderate to severe osteoarthritis (OA) of the knee with and without joint effusion and to relate these findings to both the morphological changes measured by magnetic resonance imaging (MRI) and to parameters for pain and function.
The 24 OA patients fulfilled the ACR Criteria for primary OA of the knee joint and had a Lequesne Functional Index (LFI) score of 10 or more and a normal C-reactive protein (CRP). Exclusion criteria were secondary OA, inflammatory joint disease, significant OA symptoms of the other knee or co-morbidity. ICAM-1 and VCAM-1 concentrations in plasma and SF were quantified by validated ELISA kits. Synovitis, bone marrow lesions (BML) and effusion were estimated by MRI. The Western Ontario and McMaster Universities (WOMAC) index and LFI were performed, pain was estimated by visual analogue scale (VAS) and effusion was clinically assessed by presence or absence of ballottable patella sign (BPS).
Levels of plasma ICAM-1 (median 234.47 ng/mL, range 143.92338.82) and SF ICAM-1 (median 99.78 ng/mL, range 35.06282.16) were positively correlated (p < 0.0001), (r = 0.73). Levels of ICAM-1 in SF differed between patients with and without PBS (p = 0.012). SF ICAM-1 correlated positively to MRI effusion (p = 0.009, r = 0.54), MRI synovitis (max) (p = 0.004, r = 0.59) and MRI synovitis (sum) (p = 0.003, r = 0.61). Plasma VCAM-1 (median 913.67ng/mL, range 509.811655.76) and SF VCAM (median 852.48 ng/mL, range 273.11710.01) did not correlate. SF VCAM-1 correlated positively to MRI effusion (p = 0.041, r = 0.44), MRI synovitis (max) (p = 0.041, r = 0.43) and MRI synovitis (sum) (p = 0.025, r = 0.48). SF VCAM-1 did not correlate to other parameters.
Our main finding in moderate to severe OA of the knee was that SF ICAM-1 and VCAM-1 correlated to the degree of synovitis measured by MRI. The plasma levels of ICAM-1 and VCAM-1 differed significantly between controls, OA patients without joint effusion and OA patients with joint effusion. Patients with joint effusions had the highest plasma concentration of ICAM-1 and VCAM-1.
In patients with moderate to severe knee OA and joint effusion; the concentrations of ICAM-1 and VCAM-1 in SF correlates to the degree of synovitis and effusion measured by MRI, which suggests a possible pathogenetic role for ICAM-1 and VCAM-1.
To cite this abstract, please use the following information:
Andersen, Signe E.B., Jorgensen, Annette, Ellingsen, Torkell, Egund, Niels, Stengaard-Pedersen, Kristian; ICAM-1 and VCAM-1 Concentrations in Synovial Fluid Correlate to the Degree of Synovitis Measured by MRI in Moderate to Severe Osteoarthritis of the Knee [abstract]. Arthritis Rheum 2009;60 Suppl 10 :198